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Study summary

Comparative efficacy of insect repellents against mosquito bites (complete-protection-time study)

Fradin MS, Day JF

Read the original paper
Study type
Lab
Year
2002
Sample size
15 subjects
Species tested
Aedes aegypti
Published in
New England Journal of Medicine 347(1):13-18
Evidence strength
Definitive evidence

How it was tested

Arm-in-cage complete-protection-time (CPT) tests: 15 volunteers inserted a treated arm into a cage of unfed Aedes aegypti at intervals until the first confirmed bite.

What it measured

Measured complete protection time, by arm

DEET · 23.8% · Aedes aegypti5.0 h
DEET (controlled release) · 20% · Aedes aegypti3.9 h
DEET · 6.65% · Aedes aegypti1.9 h
Soybean oil · 2% · Aedes aegypti1.6 h
DEET · 4.75% · Aedes aegypti1.5 h
IR3535 · 7.5% · Aedes aegypti22.9 min
Citronella oil · 10% · Aedes aegypti19.7 min
Citronella oil (with peppermint) · 12% · Aedes aegypti19 min
Citronella oil · 5% · Aedes aegypti13.5 min
DEET wristband · 9.5% · Aedes aegypti0.3 min

Wearable format — essentially no protection.

Complete protection time = minutes until the first confirmed bite.

Summary

The landmark complete-protection-time study: Fradin and Day timed how long 16 store-bought repellents kept Aedes aegypti from biting, in controlled arm-in-cage tests with 15 volunteers. It is the study that put hard numbers on the idea that DEET lasts and botanicals do not.

Key findings

Protection tracked DEET and its concentration above all else. A 23.8% DEET spray lasted about 5 hours (301 minutes), 20% controlled-release DEET about 4 hours, and lower-concentration DEET proportionally less. The only non-DEET product that came close was a 2% soybean-oil repellent at about 95 minutes; every citronella product lasted roughly 10 to 20 minutes, IR3535 about 23 minutes, and all three repellent wristbands gave essentially zero protection. Each step up in DEET concentration bought significantly more time.

Limitations

One mosquito species in one indoor lab, so it misses wind, sweat, and field biting pressure; the fixed-interval testing can slightly overestimate short-acting products; and the study had industry funding, though the authors report the analysis was done independently.

Related on this site

Read the original paper and see its figures ↗